High-Throughput Mapping of Single-Neuron Projections by Sequencing of Barcoded RNA.
PMID:27545715
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IF: 18.688
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Cited by: 241
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Abstract

Neurons transmit information to distant brain regions via long-range axonal projections. In the mouse, area-to-area connections have only been systematically mapped using bulk labeling techniques, which obscure the diverse projections of intermingled single neurons. Here we describe MAPseq (Multiplexed Analysis of Projections by Sequencing), a technique that can map the projections of thousands or even millions of single neurons by labeling large sets of neurons with random RNA sequences ("barcodes"). Axons are filled with barcode mRNA, each putative projection area is dissected, and the barcode mRNA is extracted and sequenced. Applying MAPseq to the locus coeruleus (LC), we find that individual LC neurons have preferred cortical targets. By recasting neuroanatomy, which is traditionally viewed as a problem of microscopy, as a problem of sequencing, MAPseq harnesses advances in sequencing technology to permit high-throughput interrogation of brain circuits.

Keywords

Anatomic
MAPseq

MeSH terms

Animals
Brain Mapping
Cerebral Cortex
High-Throughput Nucleotide Sequencing
Locus Coeruleus
Mice
Neural Pathways
Neuroanatomical Tract-Tracing Techniques
Neurons
RNA
Sequence Analysis, RNA

Authors

Kebschull, Justus M
Garcia da Silva, Pedro
Reid, Ashlan P
Peikon, Ian D
Albeanu, Dinu F
Zador, Anthony M

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