Spatially mapping the immune landscape of melanoma using imaging mass cytometry.
IF: 30.630
Cited by: 36


Melanoma is an immunogenic cancer with a high response rate to immune checkpoint inhibitors (ICIs). It harbors a high mutation burden compared with other cancers and, as a result, has abundant tumor-infiltrating lymphocytes (TILs) within its microenvironment. However, understanding the complex interplay between the stroma, tumor cells, and distinct TIL subsets remains a substantial challenge in immune oncology. To properly study this interplay, quantifying spatial relationships of multiple cell types within the tumor microenvironment is crucial. To address this, we used cytometry time-of-flight (CyTOF) imaging mass cytometry (IMC) to simultaneously quantify the expression of 35 protein markers, characterizing the microenvironment of 5 benign nevi and 67 melanomas. We profiled more than 220,000 individual cells to identify melanoma, lymphocyte subsets, macrophage/monocyte, and stromal cell populations, allowing for in-depth spatial quantification of the melanoma microenvironment. We found that within pretreatment melanomas, the abundance of proliferating antigen-experienced cytotoxic T cells (CD8+CD45RO+Ki67+) and the proximity of antigen-experienced cytotoxic T cells to melanoma cells were associated with positive response to ICIs. Our study highlights the potential of multiplexed single-cell technology to quantify spatial cell-cell interactions within the tumor microenvironment to understand immune therapy responses.


Gene Expression


Moldoveanu, Dan
Ramsay, LeeAnn
Lajoie, Mathieu
Anderson-Trocme, Luke
Lingrand, Marine
Berry, Diana
Perus, Lucas J M
Wei, Yuhong
Moraes, Cleber
Alkallas, Rached
Rajkumar, Shivshankari
Zuo, Dongmei
Dankner, Matthew
Xu, Eric Hongbo
Bertos, Nicholas R
Najafabadi, Hamed S
Gravel, Simon
Costantino, Santiago
Richer, Martin J
Lund, Amanda W
Del Rincon, Sonia V
Spatz, Alan
Miller, Wilson H Jr
Jamal, Rahima
Lapointe, Réjean
Mes-Masson, Anne-Marie
Turcotte, Simon
Petrecca, Kevin
Dumitra, Sinziana
Meguerditchian, Ari-Nareg
Richardson, Keith
Tremblay, Francine
Wang, Beatrice
Chergui, May
Guiot, Marie-Christine
Watters, Kevin
Stagg, John
Quail, Daniela F
Mihalcioiu, Catalin
Meterissian, Sarkis
Watson, Ian R

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