Three-dimensional imaging mass cytometry for highly multiplexed molecular and cellular mapping of tissues and the tumor microenvironment.

PMID:35121992

IF: 23.177

Cited by: 62

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Abstract

A holistic understanding of tissue and organ structure and function requires the detection of molecular constituents in their original three-dimensional (3D) context. Imaging mass cytometry (IMC) enables simultaneous detection of up to 40 antigens and transcripts using metal-tagged antibodies but has so far been restricted to two-dimensional imaging. Here we report the development of 3D IMC for multiplexed 3D tissue analysis at single-cell resolution and demonstrate the utility of the technology by analysis of human breast cancer samples. The resulting 3D models reveal cellular and microenvironmental heterogeneity and cell-level tissue organization not detectable in two dimensions. 3D IMC will prove powerful in the study of phenomena occurring in 3D space such as tumor cell invasion and is expected to provide invaluable insights into cellular microenvironments and tissue architecture.

Keywords

IMC

MeSH terms

Antibodies

Breast Neoplasms

Female

Humans

Image Cytometry

Imaging, Three-Dimensional

Tumor Microenvironment

Authors

Kuett, Laura

Catena, Raúl

Özcan, Alaz

Plüss, Alex

Schraml, Peter

Moch, Holger

de Souza, Natalie

Bodenmiller, Bernd

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