Classification of mouse B cell types using surfaceome proteotype maps.
Abstract
System-wide quantification of the cell surface proteotype and identification of extracellular glycosylation sites is challenging when samples are limited. Here, we miniaturize and automate the previously described Cell Surface Capture (CSC) technology, increasing sensitivity, reproducibility and throughput. We use this technology, which we call autoCSC, to create population-specific surfaceome maps of developing mouse B cells and use targeted flow cytometry to uncover developmental cell subpopulations.
Keywords
Omics
MeSH terms
Animals
B-Lymphocyte Subsets
Cell Differentiation
Cell Membrane
Flow Cytometry
HT29 Cells
High-Throughput Screening Assays
Humans
Membrane Proteins
Mice
Primary Cell Culture
Proteomics
Reproducibility of Results
Authors
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