METAHCC: Metastatic Evolution and Temporal-spatial Atlas of HepatoCellular Carcinoma(Dataset ID: STDS0000387)

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Summary
Visualization
Data
Catalog
Dataset information
Contributors
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How to cite
Dataset information
Summary:
The mechanisms underlying the interaction between disseminated tumor cells (DTCs) and their tissue microenvironment during metastasis colonization are poorly understood. We integrated multimodal single-cell and spatial profiling from liver cancer mouse models and human metastases to track the spatiotemporal dynamics of DTCs and their microenvironments across nine sequential time points from single-cell seeding until overt lung metastasis.
Overall design:
We established a stable model of lung metastasis in immunocompetent mice by intravenously injecting mCherry-labeled Hepa 1-6 murine hepatocellular carcinoma (HCC) cells (derived from a female mouse) into male C57BL6/J mice. Whole lungs were collected at nine post-injection time points spanning from initial cancer cell seeding to metastatic outgrowth: T1 (0.25 h), T2 (1 h), T3 (4 h), T4 (12 h), T5 (24 h), T6 (2 days), T7 (8 days), T8 (21 days), and T9 (35 days). At each time point, maximal right lung cross-sections were cryosectioned at 10 µm thickness to capture a roughly single-cell layer of tissue for spatial enhanced resolution omics-sequencing (Stereo-seq).
Platform:
DNBSEQ-T1
Tissues:
Lung
Sex:
Male
Disease:
lung metastases from hepatocellular carcinoma
Submission date: 2025-06-10Update date: 2025-06-10
Sample number: 18Section number: 26
DOI: To be continue
Contributors
Yu Zhong|Shang Liu
Accessions
CNSA project: CNP0005341
How to cite
  • Cite database of STOmicsDB:
    [1] Xu, Zhicheng et al. "STOmicsDB: a comprehensive database for spatial transcriptomics data sharing, analysis and visualization." Nucleic acids research vol. 52,D1 (2024): D1053-D1061. doi: 10.1093/nar/gkad933'
  • Cite visualization dataset:
    [2] Yu Zhong|Shang Liu. METAHCC: Metastatic Evolution and Temporal-spatial Atlas of HepatoCellular Carcinoma[DS/OL]. STOmicsDB, 2025[2025-06-10]. https://db.cngb.org/stomics/datasets/STDS0000387/. doi: xxxxxx
    #Format: {contributors}. {title}[DS/OL]. STOmicsDB, {the year of submission data}[{submission data}]. {dataset link}. doi: {doi ID}
  • Cite original data article:
    No results.