High-definition spatial transcriptomics for in situ tissue profiling.
Abstract
Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.
Keywords
Spatial Transcriptomics
HDST
MeSH terms
Animals
Breast Neoplasms
Female
Gene Expression Profiling
Humans
Mice
Olfactory Bulb
Sequence Analysis, RNA
Single-Cell Analysis
Tissue Array Analysis
Transcriptome
Authors
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