PMID- 31501547 OWN - NLM STAT- MEDLINE VI - 16 IP - 10 TI - High-definition spatial transcriptomics for in situ tissue profiling. PG - 987-990 LA - eng PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PL - United States TA - Nat Methods JT - Nature methods JID - 101215604 IS - 1548-7105 (Electronic) LID - 10.1038/s41592-019-0548-y [doi] FAU - Vickovic, Sanja AU - Vickovic S AUID- ORCID: http://orcid.org/0000-0003-0985-9885 AD - Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA. vickovic@broadinstitute.org. AD - Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. vickovic@broadinstitute.org. FAU - Eraslan, Gökcen AU - Eraslan G AUID- ORCID: http://orcid.org/0000-0001-9579-2909 AD - Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA. FAU - Salmén, Fredrik AU - Salmén F AD - Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. FAU - Klughammer, Johanna AU - Klughammer J AUID- ORCID: http://orcid.org/0000-0002-3628-9278 AD - Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA. FAU - Stenbeck, Linnea AU - Stenbeck L AUID- ORCID: http://orcid.org/0000-0002-0210-7886 AD - Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. FAU - Schapiro, Denis AU - Schapiro D AD - Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA. AD - Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA, USA. FAU - Äijö, Tarmo AU - Äijö T AUID- ORCID: http://orcid.org/0000-0002-3008-4634 AD - Center for Computational Biology, Flatiron Institute, New York, NY, USA. FAU - Bonneau, Richard AU - Bonneau R AD - Center for Data Science, New York University, New York, NY, USA. AD - Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA. FAU - Bergenstråhle, Ludvig AU - Bergenstråhle L AUID- ORCID: http://orcid.org/0000-0002-5108-4481 AD - Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. FAU - Navarro, José Fernandéz AU - Navarro JF AUID- ORCID: http://orcid.org/0000-0002-4035-5258 AD - Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. FAU - Gould, Joshua AU - Gould J AUID- ORCID: http://orcid.org/0000-0001-9581-5461 AD - Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA. FAU - Griffin, Gabriel K AU - Griffin GK AUID- ORCID: http://orcid.org/0000-0002-4042-6757 AD - Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA. AD - Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA. FAU - Borg, Åke AU - Borg Å AUID- ORCID: http://orcid.org/0000-0002-5793-132X AD - Division of Oncology and Pathology, Department of Clinical Sciences Lund, Lund University, Lund, Sweden. FAU - Ronaghi, Mostafa AU - Ronaghi M AD - Illumina, Inc., San Diego, CA, USA. FAU - Frisén, Jonas AU - Frisén J AD - Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden. FAU - Lundeberg, Joakim AU - Lundeberg J AD - Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. joakim.lundeberg@scilifelab.se. AD - Department of Bioengineering, Stanford University, Stanford, CA, USA. joakim.lundeberg@scilifelab.se. FAU - Regev, Aviv AU - Regev A AD - Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA. AD - Howard Hughes Medical Institute and Koch Institute for Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA. FAU - Ståhl, Patrik L AU - Ståhl PL AUID- ORCID: http://orcid.org/0000-0002-2207-7370 AD - Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. IS - 1548-7091 (Linking) SB - IM MH - Animals MH - Breast Neoplasms/pathology MH - Female MH - *Gene Expression Profiling MH - Humans MH - Mice MH - Olfactory Bulb/cytology MH - Sequence Analysis, RNA/methods MH - Single-Cell Analysis/methods MH - Tissue Array Analysis MH - *Transcriptome PMC - PMC6765407 DCOM- 20200214 LR - 20210529 DP - 201910 DEP - 20190909 AB - Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.