Imaging mass spectrometry: Molecular microscopy for the new age of biology and medicine.
Imaging mass spectrometry provides a powerful tool for monitoring and discovery of molecular processes in the spatial domain in tissues for research and practical applications in both biology and medicine. This technology directly measures molecular compounds in tissues without the use of target-specific reagents such as antibodies, is applicable to a wide variety of analytes, and can provide spatial resolutions below the single cell level. Importantly, it has paradigm shifting capabilities in clinical applications, especially for anatomic pathology.
Tissue imaging mass spectrometry
Caprioli, Richard M
1. Mass spectrometry imaging of pharmacological compounds in tissue sections.
2. Mass spectrometry imaging-based metabolomics to visualize the spatially resolved reprogramming of carnitine metabolism in breast cancer.
3. Spatially resolved metabolomics to discover tumor-associated metabolic alterations.
4. Trypsin and MALDI matrix pre-coated targets simplify sample preparation for mapping proteomic distributions within biological tissues by imaging mass spectrometry.
5. FDR-controlled metabolite annotation for high-resolution imaging mass spectrometry.