Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) imaging source coupled to a FT-ICR mass spectrometer.
PMID:23208743
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IF: 3.262
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Cited by: 100
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Abstract

Mass spectrometry imaging (MSI) allows for the direct monitoring of the abundance and spatial distribution of chemical compounds over the surface of a tissue sample. This technology has opened the field of mass spectrometry to numerous innovative applications over the past 15 years. First used with SIMS and MALDI MS that operate under vacuum, interest has grown for mass spectrometry ionization sources that allow for effective imaging but where the analysis can be performed at ambient pressure with minimal or no sample preparation. We introduce here a versatile source for MALDESI imaging analysis coupled to a hybrid LTQ-FT-ICR mass spectrometer. The imaging source offers single shot or multi-shot capability per pixel with full control over the laser repetition rate and mass spectrometer scanning cycle. Scanning rates can be as fast as 1 pixel/second and a spatial resolution of 45 μm was achieved with oversampling. Design and integration of a versatile IR-MALDESI imaging source offering multi-shot capability with a commercial FT-ICR mass spectrometer.

Keywords

SIMS
MALDI

MeSH terms

Animals
Atmospheric Pressure
Brain Chemistry
Fourier Analysis
Lipids
Lung
Mice
Molecular Imaging
Myocardium
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Authors

Robichaud, Guillaume
Barry, Jeremy A
Garrard, Kenneth P
Muddiman, David C

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