PMID- 23208743 OWN - NLM STAT- MEDLINE VI - 24 IP - 1 TI - Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) imaging source coupled to a FT-ICR mass spectrometer. PG - 92-100 LA - eng PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PL - United States TA - J Am Soc Mass Spectrom JT - Journal of the American Society for Mass Spectrometry JID - 9010412 IS - 1879-1123 (Electronic) LID - 10.1007/s13361-012-0505-9 [doi] FAU - Robichaud, Guillaume AU - Robichaud G AD - W. M. Keck FT-ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, NC, USA. FAU - Barry, Jeremy A AU - Barry JA FAU - Garrard, Kenneth P AU - Garrard KP FAU - Muddiman, David C AU - Muddiman DC IS - 1044-0305 (Linking) RN - 0 (Lipids) SB - IM MH - Animals MH - Atmospheric Pressure MH - Brain Chemistry MH - Fourier Analysis MH - Lipids/analysis/chemistry MH - Lung/chemistry MH - Mice MH - Molecular Imaging/*methods MH - Myocardium/chemistry MH - Spectrometry, Mass, Electrospray Ionization/*methods MH - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods PMC - PMC3689149 DCOM- 20130701 LR - 20211021 DP - 2013 Jan DEP - 20121204 AB - Mass spectrometry imaging (MSI) allows for the direct monitoring of the abundance and spatial distribution of chemical compounds over the surface of a tissue sample. This technology has opened the field of mass spectrometry to numerous innovative applications over the past 15 years. First used with SIMS and MALDI MS that operate under vacuum, interest has grown for mass spectrometry ionization sources that allow for effective imaging but where the analysis can be performed at ambient pressure with minimal or no sample preparation. We introduce here a versatile source for MALDESI imaging analysis coupled to a hybrid LTQ-FT-ICR mass spectrometer. The imaging source offers single shot or multi-shot capability per pixel with full control over the laser repetition rate and mass spectrometer scanning cycle. Scanning rates can be as fast as 1 pixel/second and a spatial resolution of 45 μm was achieved with oversampling. Design and integration of a versatile IR-MALDESI imaging source offering multi-shot capability with a commercial FT-ICR mass spectrometer.