In situ primary metabolites localization on a rat brain section by chemical mass spectrometry imaging.

PMID:19514699

IF: 8.008

Cited by: 86

Abstract

We describe here the detection and identification of 13 primary metabolites (AMP, ADP, ATP, UDP-GlcNAc, ...) directly from rat brain sections by chemical mass spectrometry imaging. Matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI-MS/MS) was combined with 9-aminoacridine as a powerful matrix. We also demonstrate that a new robotic sprayer allows us to homogeneously coat the surface with the matrix, enabling the acquisition of chemical images at a 50 microm resolution, leading us to precisely and simultaneously localize each metabolite over the tissue surface. These experiments open a new field of investigation for chemical mass spectrometry imaging and are of great interest for both chemists and biologists.

Keywords

MALDI

MeSH terms

Aminacrine

Animals

Brain

Metabolome

Rats

Spectrometry, Mass, Electrospray Ionization

Tandem Mass Spectrometry

Authors

Benabdellah, Farida

Touboul, David

Brunelle, Alain

Laprévote, Olivier

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