PMID- 19514699 OWN - NLM STAT- MEDLINE VI - 81 IP - 13 TI - In situ primary metabolites localization on a rat brain section by chemical mass spectrometry imaging. PG - 5557-60 LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Anal Chem JT - Analytical chemistry JID - 0370536 IS - 1520-6882 (Electronic) LID - 10.1021/ac9005364 [doi] FAU - Benabdellah, Farida AU - Benabdellah F AD - Institut de Chimie des Substances Naturelles, CNRS UPR 2301, Avenue de la Terrasse 91198 Gif-sur-Yvette Cedex, France. FAU - Touboul, David AU - Touboul D FAU - Brunelle, Alain AU - Brunelle A FAU - Laprévote, Olivier AU - Laprévote O IS - 0003-2700 (Linking) RN - 78OY3Z0P7Z (Aminacrine) SB - IM MH - Aminacrine/*chemistry MH - Animals MH - Brain/*metabolism MH - *Metabolome MH - Rats MH - Spectrometry, Mass, Electrospray Ionization/*methods MH - Tandem Mass Spectrometry/*methods DCOM- 20090929 LR - 20131121 DP - 2009 Jul 01 AB - We describe here the detection and identification of 13 primary metabolites (AMP, ADP, ATP, UDP-GlcNAc, ...) directly from rat brain sections by chemical mass spectrometry imaging. Matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI-MS/MS) was combined with 9-aminoacridine as a powerful matrix. We also demonstrate that a new robotic sprayer allows us to homogeneously coat the surface with the matrix, enabling the acquisition of chemical images at a 50 microm resolution, leading us to precisely and simultaneously localize each metabolite over the tissue surface. These experiments open a new field of investigation for chemical mass spectrometry imaging and are of great interest for both chemists and biologists.