Single-cell gene expression profiling.

IF: 63.714

Cited by: 413

Abstract

A key goal of biology is to relate the expression of specific genes to a particular cellular phenotype. However, current assays for gene expression destroy the structural context. By combining advances in computational fluorescence microscopy with multiplex probe design, we devised technology in which the expression of many genes can be visualized simultaneously inside single cells with high spatial and temporal resolution. Analysis of 11 genes in serum-stimulated cultured cells revealed unique patterns of gene expression within individual cells. Using the nucleus as the substrate for parallel gene analysis, we provide a platform for the fusion of genomics and cell biology: "cellular genomics."

Keywords

Omics

Cellular Genomics

Gene Expression

MeSH terms

Adenocarcinoma

Cell Nucleus

Cells

Colonic Neoplasms

Color

DNA Probes

Fibroblasts

Gene Expression Profiling

Gene Expression Regulation

Genes

Genomics

Humans

Microscopy, Fluorescence

Odds Ratio

RNA

Sensitivity and Specificity

Time Factors

Transcription, Genetic

Tumor Cells, Cultured

Authors

Levsky, Jeffrey M

Shenoy, Shailesh M

Pezo, Rossanna C

Singer, Robert H

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