PMID- 12161654 OWN - NLM STAT- MEDLINE VI - 297 IP - 5582 TI - Single-cell gene expression profiling. PG - 836-40 LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Science JT - Science (New York, N.Y.) JID - 0404511 IS - 1095-9203 (Electronic) FAU - Levsky, Jeffrey M AU - Levsky JM AD - Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA. FAU - Shenoy, Shailesh M AU - Shenoy SM FAU - Pezo, Rossanna C AU - Pezo RC FAU - Singer, Robert H AU - Singer RH IS - 0036-8075 (Linking) RN - 0 (DNA Probes) RN - 63231-63-0 (RNA) SB - IM MH - Adenocarcinoma/genetics MH - Cell Nucleus/genetics MH - Cells/*cytology/*metabolism MH - Colonic Neoplasms/genetics MH - Color MH - DNA Probes MH - Fibroblasts MH - Gene Expression Profiling/instrumentation/*methods MH - *Gene Expression Regulation MH - Genes MH - Genomics/instrumentation/methods MH - Humans MH - Microscopy, Fluorescence/instrumentation/*methods MH - Odds Ratio MH - RNA/genetics/metabolism MH - Sensitivity and Specificity MH - Time Factors MH - Transcription, Genetic MH - Tumor Cells, Cultured DCOM- 20020820 LR - 20210218 DP - 2002 Aug 02 AB - A key goal of biology is to relate the expression of specific genes to a particular cellular phenotype. However, current assays for gene expression destroy the structural context. By combining advances in computational fluorescence microscopy with multiplex probe design, we devised technology in which the expression of many genes can be visualized simultaneously inside single cells with high spatial and temporal resolution. Analysis of 11 genes in serum-stimulated cultured cells revealed unique patterns of gene expression within individual cells. Using the nucleus as the substrate for parallel gene analysis, we provide a platform for the fusion of genomics and cell biology: "cellular genomics."