STOmics technology:BGI Stereomics Stereo-Seq

Data type:Spatial transcriptomics

Sample scope:Monoisolate

Summary:The contribution of migrating cardiac neural crest cell (CNCC) to the cardiovascular system is a dynamic and finely regulated process. However, the environmental signals influencing when, where, and how CNCCs migrate and begin to differentiate remain to be fully elucidated. In this study, we combined scRNA-seq and scStereo-seq to construct a spatiotemporal atlas of early developmental mouse cardiopharyngeal. We revealed three distinct transition paths and associated biological events during early NCC mesenchymalization, which are related to pre-epithelial-mesenchymal transition and early migration NCCs and their migratory environments. Notably, different transition paths appear to correspond to different downstream fates including CNCC and chondrocyte. Furthermore, we assigned Gata3+ cardiac mesenchymal cells in the pharyngeal region to the Sox10+ migration NCC lineage, categorizing them into distinct subtypes contributing to the outflow tract and pharyngeal arches, and reconstructed their spatiotemporal molecular dynamics of migration and differentiation. Our analysis also indicates that CNCCs migrating to the endocardial cushions and endocardial cells acquired similar mesenchymal fates during early valve development. Additionally, interactions between CNCCs and non-NCC lineage cells facilitated CNCC specification and tissue remodeling. In summary, our study provides new insights into the spatiotemporal lineage evolution and regulatory mechanisms of CNCCs

Contributor(s):Zhao Zhang.

Submitter:张钊,BGI group

Release date:2025-06-01

Updated:2025-06-01