STOmics technology:BGI Stereomics Stereo-Seq

Data type:Spatial transcriptomics

Sample scope:Monoisolate

Summary:Septic encephalopathy, recognized for its considerable morbidity and mortality rates, poses a substantial challenge in understanding its pathogenesis. This study utilized a murine model where lipopolysaccharide (LPS) was intraperitoneally administered to both wild type and Anxa1-/- mice. Employing single-cell sequencing and Stereo-seq technology, we analyzed brain tissues at multiple time points post-LPS injection to elucidate the dynamic cellular and molecular responses. Our findings revealed a time-dependent increase in activated glial cells and perivascular aggregation, along with the upregulation of specific receptor ligands following LPS administration. This temporal pattern underscores the dynamic nature of the inflammatory response in septic encephalopathy, irrespective of the presence of the Anxa1. Conversely, Anxa1-/- mice resulted in an earlier decrease in activated astrocytes and receptor ligands compared to wild type mice. These results highlight the pivotal role of temporal changes in driving the disease progression, with the effects of Anxa1 deletion manifesting in a nuanced manner.

Contributor(s):Xiaodong Fang, Pingsen Zhao, Yin Zhang, Yanyan Zhu.

Submitter:张银(Yin Zhang),BGI Research at Shenzhen

Release date:2025-05-06

Updated:2025-05-06