Spatiotemporal immune atlas of a clinical-grade gene-edited pig-to-human kidney xenotransplant(Dataset ID: STDS0000361)

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Summary
Data
Sample
Catalog
Dataset information
Contributors
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How to cite
Dataset information
Summary:
Pig-to-human xenotransplantation is rapidly approaching the clinical arena; however, it is unclear which immunomodulatory regimens will effectively control human immune responses to pig xenografts. Here, we transplant a gene-edited pig kidney into a brain-dead human recipient on pharmacologic immunosuppression and study the human immune response to the xenograft using spatial transcriptomics and single-cell RNA sequencing. Human immune cells are uncommon in the porcine kidney cortex early after xenotransplantation and consist of primarily myeloid cells. Both the porcine resident macrophages and human infiltrating macrophages express genes consistent with an alternatively activated, anti-inflammatory phenotype. No significant infiltration of human B or T cells into the porcine kidney xenograft is detectable. Altogether, these findings provide proof of concept that conventional pharmacologic immunosuppression is sufficient to restrict infiltration of human immune cells into the xenograft early after compatible pig-to-human kidney xenotransplantation.
Overall design:
Wild-type pig and human kidneys were procured. Porcine kidney biopsies were flash frozen in optimal cutting temperature, then processed and analyzed using the 10X Genomics Visium Spatial Transcriptomics platform; Please note that the C9WTB* processed data is generated from all Wild Type Pig Kidney_L* samples and is linked to the Wild Type Pig Kidney_L1 sample records.
Technology:
ST
Platform:
Illumina NovaSeq 6000, Illumina NovaSeq 6000
Species:
Homo sapiens
Sus scrofa
Tissues:
Kidney
Submission date: 2023-09-04Update date: 2024-05-01
DOI: To be continue
Contributors
Asiimwe, Rebecca; Cheung, Matthew D; Erman, Elise N; Fucile, Christopher F; Liu, Shanrun; Sun, Chiao-Wang; Hanumanthu, Vidya S; Pal, Harish C; Wright, Emma D; Ghajar-Rahimi, Gelare; Epstein, Daniel; Orandi, Babak J; Kumar, Vineeta; Anderson, Douglas J; Greene, Morgan E; Bell, Markayla; Yates, Stefani; Moore, Kyle H; LaFontaine, Jennifer; Killian Jr., John T; Baker, Gavin; Perry, Jackson; Khan, Zayd; Reed, Rhiannon; Little, Shawn C; Rosenberg, Alexander F; George, James F; Locke, Jayme E; Porrett, Paige M
Contact: contrib1
Accessions
GEO Series Accessions: GSE242270
How to cite
  • Cite database of STOmicsDB:
    [1] Xu, Zhicheng et al. "STOmicsDB: a comprehensive database for spatial transcriptomics data sharing, analysis and visualization." Nucleic acids research vol. 52,D1 (2024): D1053-D1061. doi: 10.1093/nar/gkad933'
  • Cite visualization dataset:
    [2] Asiimwe, Rebecca. Spatiotemporal immune atlas of a clinical-grade gene-edited pig-to-human kidney xenotransplant[DS/OL]. STOmicsDB, 2023[2023-09-04]. https://db.cngb.org/stomics/datasets/STDS0000361/. doi: xxxxxx
    #Format: {contributors}. {title}[DS/OL]. STOmicsDB, {the year of submission data}[{submission data}]. {dataset link}. doi: {doi ID}
  • Cite original data article:
    No results.