Spatial gene expression of canine veins during carotid-cartoid vein bypass implantation(Dataset ID: STDS0000253)

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Spots: 4,903
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Genes: 35,555
Summary
Sample
Catalog
Dataset information
Contributors
Accessions
How to cite
Dataset information
Summary:
Vein graft failure (VGF) following cardiovascular bypass surgery results in significant patient morbidity and cost to the healthcare system. Vein graft injury can occur during autogenous vein harvest and preparation, as well as after implantation into the arterial system, leading to the development of intimal hyperplasia, vein graft stenosis, and, ultimately, bypass graft failure. While previous studies have identified maladaptive pathways that occur shortly after implantation, the specific signaling pathways that occur during vein graft preparation are not well defined and may result in a cumulative impact on VGF. We, therefore, aimed to elucidate the response of the vein conduit wall during harvest and following implantation, probing the key maladaptive pathways driving graft failure with the overarching goal of identifying therapeutic targets for biologic intervention to minimize these natural responses to surgical vein graft injury. Employing a novel approach to investigating vascular pathologies, we harnessed both single-nuclei RNA-sequencing (snRNA-seq) and spatial transcriptomics (ST) analyses to profile the genomic effects of vein grafts after harvest and distension, then compared these findings to vein grafts obtained 24 hours after carotid-cartoid vein bypass implantation in a canine model (n=4). Collectively, we find that vein conduit harvest and distension elicit a prompt genomic response facilitated by distinct cellular subpopulations heterogeneously distributed throughout the vein wall. This response was found to be further exacerbated following vein graft implantation, resulting in a cascade of maladaptive gene regulatory networks. Together, these results suggest that distension initiates the upregulation of pathological pathways that may ultimately contribute to bypass graft failure and presents potential early targets warranting investigation for targeted therapies.
Overall design:
Bilateral upper limb cephalic vein harvest and carotid-cartoid vein bypass implantation were performed on 25 kg male (n=3) or female (n=1) mongrel dogs. Samples (15 cm) of distended vein were taken following distension during graft harvesting and at 24 hours post-bypass, alongside untouched control vein samples, and embedded in OCT blocks and flash-frozen in an isopentane and liquid nitrogen bath for spatial transcriptomics analysis.
Technology:
ST
Platform:
Illumina NovaSeq 6000
Species:
Canis lupus familiaris
Tissues:
Cephalic vein
Sex:
F/M
Submission date: 2024-04-05Update date: 2024-05-30
Sample number: 8Section number: 8
DOI: To be continue
Contributors
Michaud, Marina; Marina, Michaud; Lucas, Mota; Patric, Liang; Manoj, Bhasin
Contact: contrib1
Accessions
GEO Series Accessions: GSE263281
How to cite
  • Cite database of STOmicsDB:
    [1] Xu, Zhicheng et al. "STOmicsDB: a comprehensive database for spatial transcriptomics data sharing, analysis and visualization." Nucleic acids research vol. 52,D1 (2024): D1053-D1061. doi: 10.1093/nar/gkad933'
  • Cite visualization dataset:
    [2] Michaud, Marina. Spatial gene expression of canine veins during carotid-cartoid vein bypass implantation[DS/OL]. STOmicsDB, 2024[2024-04-05]. https://db.cngb.org/stomics/datasets/STDS0000253/. doi: xxxxxx
    #Format: {contributors}. {title}[DS/OL]. STOmicsDB, {the year of submission data}[{submission data}]. {dataset link}. doi: {doi ID}
  • Cite original data article:
    No results.