MPSTA: Mouse Placentation Spatiotemporal Transcriptomic Atlas(Dataset ID: STDS0000245)

0
|
118
|
1,136
Summary
Visualization
Data
Catalog
Dataset information
Contributors
Accessions
How to cite
Dataset information
Summary:
The intrauterine environment, composed of the placenta, fetal membranes, and the maternal uterus, undergoes intricate and significant changes during embryonic development. However, it has not been systematically characterized in a spatial view, which limit our understanding of placental development and maternal-fetal diseases. Here, we used Stereo-seq to establish the comprehensive transcriptome landscapes on 13 segments of the mouse uterus at 6 different time points ranging from E7.5 to E14.5, integrated with previously published scRNA-seq data. We identified the specific regional composition of the ectoplacental cone and elucidated the spatial trajectory of trophoblast differentiation during early post-implantation stages. Our atlas of the intrauterine environment revealed interface-specific TFs at the early interface of placental invasion and uncovered spatially restricted communications in the labyrinth. Furthermore, we revealed the distinctive spatial distribution of maternal and paternal imprinting genes, as well as genes associated with heart development, providing valuable spatial datasets especially for congenital heart disease. Importantly, our study demonstrated that maternal high-fat diet, as an adverse intrauterine exposure, upregulates inflammatory responses and disrupts angiogenesis regulation in the interface of placental invasion. Overall, this study has established the most systematic spatiotemporal single-cell resolution transcriptome atlas of the intrauterine environment placental development following embryo implantation.
Overall design:
MPSTA database has a total of 15 sagittal sections from C57BL/6 mouse uterine at E7.5 (x2), E8.5 (x2), E9.5 (x2), E10.5 (x3), E10.5 HFD(x1), E12.5 (x2), E14.5 (x2), E14.5 HFD (x1) using Stereo-seq. For each stages, at least two sections were included.
Platform:
DNBSEQ-T1
Tissues:
uterine
Development stage:
E7.5 | E8.5 | E9.5 | E10.5 | E12.5 | E14.5 | all
Disease:
Normal vs. HFD (high-fat diet) group
Submission date: 2023-11-14Update date: 2025-05-20
Sample number: 18Section number: 18
DOI: To be continue
Contributors
Ying Zhang
Accessions
CNSA project: CNP0004966
STOmicsDB: STT0000055
How to cite
  • Cite database of STOmicsDB:
    [1] Xu, Zhicheng et al. "STOmicsDB: a comprehensive database for spatial transcriptomics data sharing, analysis and visualization." Nucleic acids research vol. 52,D1 (2024): D1053-D1061. doi: 10.1093/nar/gkad933'
  • Cite visualization dataset:
    [2] Ying Zhang. MPSTA: Mouse Placentation Spatiotemporal Transcriptomic Atlas[DS/OL]. STOmicsDB, 2023[2023-11-14]. https://db.cngb.org/stomics/datasets/STDS0000245/. doi: xxxxxx
    #Format: {contributors}. {title}[DS/OL]. STOmicsDB, {the year of submission data}[{submission data}]. {dataset link}. doi: {doi ID}
  • Cite original data article:
    Citation: Wu et al. “Spatiotemporal transcriptomic atlas of mouse placentation.”Under Review