Cholestatic Injury and Repair Spatio-Temporal Atlas(Dataset ID: STDS0000239)

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Genes: 24,531
Summary
Visualization
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Dataset information
Summary:
Cholestatic injuries, characterized by regional damage around the periportal region, lack curative therapies and cause considerable mortality. In this study, we generated a high-definition spatiotemporal atlas during cholestatic injury and repair by Stereo-seq and single-cell transcriptomics. We uncovered that cholangiocytes function as a periportal hub (cholangio-hub) by integrating multiple signals with neighboring cells. Feedback between cholangiocytes and lipid-associated macrophages (LAM) was detected in the cholangio-hub, which is related to the differentiation of LAM, a recently identified subpopulation of macrophages crucial in tissue injury. Moreover, the cholangio-hub highly expressed TGFβ, which is associated with cholangiocyte conversion of liver progenitor-like cells during injury and dampened proliferation of periportal hepatocytes during recovery. Importantly, spatiotemporal analysis revealed a key inhibitory rheostat for hepatocyte proliferation. Our data provide a comprehensive resource for demarcating regional cholestatic injuries.
Overall design:
We collected C57BL/6 mouse livers during DDC injury and withdrawal at six time points, 107 normal livers (day 0 before DDC treatment, D0), DDC-damaged livers at days 8 and 108 17 (D8 and D17), livers at recovery days 2, 7 and 21 (R2, R7, and R21)
Technology:
Stereo-Seq
Platform:
DNBseq
Species:
Mus musculus
Tissues:
Liver
Cell types:
Hepatocyte,Cholangiocyte,Monocyte,Macrophage,Fibroblast,Hepatic Stellate cell,Kupffer,Liver Sinusoid
Submission date: 2024-03-22Update date: 2024-03-22
Sample number: 50Section number: 22
DOI: To be continue
Contributors
Shijie Hao
Contact: haoshijie@genomics.cn
Accessions
No results.
How to cite
  • Cite database of STOmicsDB:
    [1] Xu, Zhicheng et al. "STOmicsDB: a comprehensive database for spatial transcriptomics data sharing, analysis and visualization." Nucleic acids research vol. 52,D1 (2024): D1053-D1061. doi: 10.1093/nar/gkad933'
  • Cite visualization dataset:
    [2] Shijie Hao. Cholestatic Injury and Repair Spatio-Temporal Atlas[DS/OL]. STOmicsDB, 2024[2024-03-22]. https://db.cngb.org/stomics/datasets/STDS0000239/. doi: xxxxxx
    #Format: {contributors}. {title}[DS/OL]. STOmicsDB, {the year of submission data}[{submission data}]. {dataset link}. doi: {doi ID}
  • Cite original data article:
    No results.