Geography and molecular anatomy of germ layers in the gastrulating primate embryo
chevron_leftchevron_right
Catalog
Dataset information
Contributors
Accessions
Dataset information
Summary:
During mammalian embryogenesis, temporal and spatial regulation of gene expression and cell signaling influences lineage specification, the patterning of tissue progenitors and the morphogenesis of embryo. While the mouse model has been instrumental for our understanding of mammalian development, comparatively little is known about early human and non-human primate gastrulation due to the limitation of technical and ethical. Here, we present a morphological and molecular approach that reveals the systematically morphological changes and comprehensive transcriptional landscape of cell types populating the non-human primate embryos during gastrulation.Overall design:
By using spatial transcriptome of Geo-seq, we carried out transcriptome profiling on Crab-eating macaque embryo sections at a high resolution of 5-50 cells per sample. We then constructed a comprehensive spatial transcriptome map in gastrulating embryos.Technology:
Geo-seq
Platform:
Illumina NovaSeq 6000 (Macaca fascicularis)
Species:
Macaca fascicularis(macFas5)
Tissues:
Embryo
Organ parts:
Gastrulation embryos
Development stage:
E20
Citation:
Cui, Guizhong et al. “Spatial molecular anatomy of germ layers in the gastrulating cynomolgus monkey embryo.” Cell reports vol. 40,9 (2022): 111285. doi:10.1016/j.celrep.2022.111285Submission date: 2021-08-26Update date: 2022-09-06
Sample number: 1Section number: 1
Contributors
Cui, Guizhong; Si, Wei; Jing, Naihe; Peng, Guangdun
Accessions
GEO Series Accessions:
GSE182838