Cell types and clonal relations in the mouse brain revealed by single-cell and spatial transcriptomics(Dataset ID: STDS0000085)
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Dataset information
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Dataset information
Summary:
We report TREX for simultaneous lineage tracking and expression profiling of dissociated cells from the juvenile mouse brain using single cell transcriptomics. We also developed Space-TREX enabling tracking and transcriptome profiling in intact tissue sections via Spatial Transcriptomics.Overall design:
Lentiviral delivery of a transcribed barcode into neuroepithelial progenitor cells using in utero microinjection into the mouse forebrain at embryonic day 9.5 followed by RNA-seq analysis of barcoded cells at postnatal day 11-14.Technology:
10x Visium
Platform:
GPL16085, GPL16417, GPL21626, GPL24247
Species:
Mus musculus(mm10)
Tissues:
Brain
Development stage:
P11,P14
Submission date: 2020-06-28Update date: 2021-04-08
DOI: To be continue
Contributors
Michael Ratz,Leonie von Berlin,Ludvig Larsson,Marcel Martin,Jakub O Westholm,Gioele La Manno,Joakim
Contact: michael.ratz@ki.se
Accessions
GEO Series Accessions:
GSE153424
How to cite
- Cite database of STOmicsDB:[1] Xu, Zhicheng et al. "STOmicsDB: a comprehensive database for spatial transcriptomics data sharing, analysis and visualization." Nucleic acids research vol. 52,D1 (2024): D1053-D1061. doi: 10.1093/nar/gkad933'
- Cite visualization dataset:[2] Michael Ratz,Leonie von Berlin,Ludvig Larsson,Marcel Martin,Jakub O Westholm,Gioele La Manno,Joakim . Cell types and clonal relations in the mouse brain revealed by single-cell and spatial transcriptomics[DS/OL]. STOmicsDB, 2020[2020-06-28]. https://db.cngb.org/stomics/datasets/STDS0000085/. doi: xxxxxx#Format: {contributors}. {title}[DS/OL]. STOmicsDB, {the year of submission data}[{submission data}]. {dataset link}. doi: {doi ID}
- Cite original data article:Citation: Ratz, Michael et al. “Clonal relations in the mouse brain revealed by single-cell and spatial transcriptomics.” Nature neuroscience vol. 25,3 (2022): 285-294. doi:10.1038/s41593-022-01011-x