Cell types and clonal relations in the mouse brain revealed by single-cell and spatial transcriptomics
Dataset ID: STDS0000085
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PMID:35210624
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30,667 Spots
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27,999 Genes
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Dataset information
Contributors
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Dataset information
Summary:
We report TREX for simultaneous lineage tracking and expression profiling of dissociated cells from the juvenile mouse brain using single cell transcriptomics. We also developed Space-TREX enabling tracking and transcriptome profiling in intact tissue sections via Spatial Transcriptomics.
Overall design:
Lentiviral delivery of a transcribed barcode into neuroepithelial progenitor cells using in utero microinjection into the mouse forebrain at embryonic day 9.5 followed by RNA-seq analysis of barcoded cells at postnatal day 11-14.
Technology:
10x Visium
Platform:
GPL16085, GPL16417, GPL21626, GPL24247
Species:
Mus musculus(mm10)
Tissues:
Brain
Development stage:
P11, P14
Citation:
Ratz, Michael et al. “Clonal relations in the mouse brain revealed by single-cell and spatial transcriptomics.” Nature neuroscience vol. 25,3 (2022): 285-294. doi:10.1038/s41593-022-01011-x
Submission date: 2020-06-28Update date: 2021-04-08
Contributors
Michael Ratz; Leonie von Berlin; Ludvig Larsson; Marcel Martin; Jakub O Westholm; Gioele La Manno; Joakim Lundeberg; Jonas Frisén
Contact: michael.ratz@ki.se
Accessions
GEO Series Accessions: GSE153424