High spatial resolution multi-omics atlas sequencing of mouse embryos
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Dataset information
Summary:
Microfluidic deterministic barcoding of mRNAs and proteins in tissue slides followed by high throughput sequencing enables the construction of high-spatial-resolution multi-omics atlas at the genome scale. Applying it to mouse embryo tissues revealed major tissue (sub)types in early-stage organogenesis, brain micro-vasculatures, and the fine structure of an optical vesicle at the single-cell-layer resolution.Overall design:
Applying new approach 鈥?microfluidic Deterministic Barcoding in Tissue for spatial omics sequencing to mouse embryo tissues in different resolution (10渭m, 25渭m, 50渭m).Technology:
DBiT-seq
Platform:
Illumina HiSeq 4000
Species:
Mus musculus
Tissues:
Embryo
Development stage:
E10, E12, E11
Citation:
Liu, Yang et al. “High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue.” Cell vol. 183,6 (2020): 1665-1681.e18. doi:10.1016/j.cell.2020.10.026Submission date: 2019-09-25Update date: 2020-12-16
Contributors
Rong Fan; Yang Liu; Mingyu Yang; Yanxiang Deng
Contact: mingyu.yang@yale.edu
Accessions
GEO Series Accessions:
GSE137986