High spatial resolution multi-omics atlas sequencing of mouse embryos
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Summary
Microfluidic deterministic barcoding of mRNAs and proteins in tissue slides followed by high throughput sequencing enables the construction of high-spatial-resolution multi-omics atlas at the genome scale. Applying it to mouse embryo tissues revealed major tissue (sub)types in early-stage organogenesis, brain micro-vasculatures, and the fine structure of an optical vesicle at the single-cell-layer resolution.
Microfluidic deterministic barcoding of mRNAs and proteins in tissue slides followed by high throughput sequencing enables the construction of high-spatial-resolution multi-omics atlas at the genome scale. Applying it to mouse embryo tissues revealed major tissue (sub)types in early-stage organogenesis, brain micro-vasculatures, and the fine structure of an optical vesicle at the single-cell-layer resolution.
Overall design
Applying new approach 鈥?microfluidic Deterministic Barcoding in Tissue for spatial omics sequencing to mouse embryo tissues in different resolution (10渭m, 25渭m, 50渭m).
Applying new approach 鈥?microfluidic Deterministic Barcoding in Tissue for spatial omics sequencing to mouse embryo tissues in different resolution (10渭m, 25渭m, 50渭m).
Technology
DBiT-seq
DBiT-seq
Platform
Illumina HiSeq 4000
Illumina HiSeq 4000
Species
Mus musculus
Tissues
Embryo
Embryo
Development stage
Embryonic day 10, Embryonic day 12, Embryonic Day 11
Embryonic day 10, Embryonic day 12, Embryonic Day 11
Citation
Liu Y, Yang M, Deng Y, Su G et al. High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue. Cell 2020 Dec 10;183(6):1665-1681.e18.
Liu Y, Yang M, Deng Y, Su G et al. High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue. Cell 2020 Dec 10;183(6):1665-1681.e18.
Submission date: 2019-09-25Update date: 2020-12-16
Accessions
GEO Series Accessions:
GSE137986