LCM-Seq analysis of microscopically defined human embryo HSC microenvironment
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Dataset information
Summary
This dataset contains RNA-Seq data for cells within spatially defined subdomains of the human embryo dorsal aorta, subdissected from tissue sections using laser capture microdissection. This dataset explores dorsal-ventral polarisation across the dorsal aorta, the site of hematopoietic stem cell emergence.
This dataset contains RNA-Seq data for cells within spatially defined subdomains of the human embryo dorsal aorta, subdissected from tissue sections using laser capture microdissection. This dataset explores dorsal-ventral polarisation across the dorsal aorta, the site of hematopoietic stem cell emergence.
Overall design
In total 30 samples (subdomains) from 6 human embryos are analysed. This includes 6 subdomains (V_Inner, D_Inner, V_Mid, D_Mid, V_Outer, D_Outer) from 3 CS16 embryos and 4 subdomains ([V], [VL], [DL], [D]) from 3 CS17 embryos.
In total 30 samples (subdomains) from 6 human embryos are analysed. This includes 6 subdomains (V_Inner, D_Inner, V_Mid, D_Mid, V_Outer, D_Outer) from 3 CS16 embryos and 4 subdomains ([V], [VL], [DL], [D]) from 3 CS17 embryos.
Technology
LCM-seq
LCM-seq
Platform
Illumina HiSeq 4000, Illumina NovaSeq 6000
Illumina HiSeq 4000, Illumina NovaSeq 6000
Species
Homo sapiens
Tissues
Dorsal aorta
Dorsal aorta
Cell types
CS17, CS16
CS17, CS16
Citation
Crosse EI, Gordon-Keylock S, Rybtsov S, Binagui-Casas A et al. Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development. Cell Stem Cell 2020 Nov 5;27(5):822-839.e8.
Crosse EI, Gordon-Keylock S, Rybtsov S, Binagui-Casas A et al. Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development. Cell Stem Cell 2020 Nov 5;27(5):822-839.e8.
Submission date: 2020-06-05Update date: 2020-12-01