LCM-Seq analysis of microscopically defined human embryo HSC microenvironment
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Dataset information
Summary:
This dataset contains RNA-Seq data for cells within spatially defined subdomains of the human embryo dorsal aorta, subdissected from tissue sections using laser capture microdissection. This dataset explores dorsal-ventral polarisation across the dorsal aorta, the site of hematopoietic stem cell emergence.Overall design:
In total 30 samples (subdomains) from 6 human embryos are analysed. This includes 6 subdomains (V_Inner, D_Inner, V_Mid, D_Mid, V_Outer, D_Outer) from 3 CS16 embryos and 4 subdomains ([V], [VL], [DL], [D]) from 3 CS17 embryos.Technology:
LCM-seq
Platform:
Illumina HiSeq 4000, Illumina NovaSeq 6000
Species:
Homo sapiens(hg38)
Tissues:
Dorsal aorta
Cell types:
CS17, CS16
Citation:
Crosse, Edie I et al. “Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development.” Cell stem cell vol. 27,5 (2020): 822-839.e8. doi:10.1016/j.stem.2020.08.004Submission date: 2020-06-05Update date: 2020-12-01
Contributors
Edie I Crosse; Alexander Medvinsky
Contact: edie.crosse@outlook.com