Vitamin D sufficiency enhances differentiation of patient-derived prostate epithelial organoids
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Summary:
Spatially resolved gene expression was prepard by dissociated hman prostate tissue to single cells, and collected & prepped for RNA-seq using the Visium Spatial Gene Expression kit. 5000 cells were collected and sequenced at a depth of 50k cells/gene on a 2X150nt lane in a NovaSeq 6000. SpaceRanger alignment was performed to produce the RAW filesOverall design:
Two samples:ETOH, 25DTechnology:
10x Visium, scRNA
Platform:
Illumina NovaSeq 6000
Species:
Homo sapiens(hg38)
Tissues:
Prostate
Organ parts:
Prostate epithelial organoids
Cell types:
stem cell, dividing cells, The “IntegrinHigh”, progenitor, luminal
Citation:
McCray, Tara et al. “Vitamin D sufficiency enhances differentiation of patient-derived prostate epithelial organoids.” iScience vol. 24,1 101974. 5 Jan. 2021, doi:10.1016/j.isci.2020.101974Submission date: 2020-10-20Update date: 2021-06-19
Sample number: 2Section number: 2
Contributors
Garcia J; Loitz C; Nonn L
Contact: lnonn@uic.edu