Low-cost, high-throughput sequencing service
China National Gene Bank currently has the world's leading gene sequencing platform and the nation's leading database and corresponding information analysis platform with Pb-level annual data output capacity.All sequencing instruments used in the sequencing platform of CNGB are domestically-made sequencers, which can provide low-cost, high-throughput sequencing services for agricultural research institutions and enterprises. At the same time, in frontier fields such as single cell research, a standardized and automated single-cell sequencing platform has been built, with an annual single-cell output capacity of 30 million. The re-sequencing of 3,000 rice in 2011 took nearly one year, about 4.5 million yuan. But today, the same work can be completed within three months with 500,000 yuan.
The CNGB can provide low-cost and high-throughput sequencing services for agricultural research institutions and enterprises, quickly transform germplasm resources into germplasm gene data resources for long-term preservation, development and utilization, and create a new model of rapid breeding---"germplasm + gene".
Whole Genome Re-sequencing Analysis of Animal and Plant
The whole genome re-sequencing is to perform DNA sequencing of species with known genome sequence, and complete individual or population analysis on this basis. Whole genome resequencing can detect a large amount of variation information through sequence alignment, including single nucleotide polymorphism (SNP), insertion deletion (InDel), structural variation (SV) and copy number variation (CNV), etc. Based on the detected variation, it can further study the species characteristics of animals and plants, population evolution issues, and locate gene loci of target trait .
With the reduction of sequencing costs and the increase of species with known genome sequences, whole-genome re-sequencing has become one of the most rapid and effective methods in animal and plant molecular breeding and population evolution analysis. The whole-genome re-sequencing technology can help to quickly discover genetic variation related to important traits of animals and plants, and shorten the breeding cycle in molecular breeding.
Product Advantage
Simple technology and good stability
Various types of detection variants: multiple variant types can be detected such as SNP, InDel, SV and CNV, which can be used as molecular markers.
High-density labeling: SNPs information in the whole genome can be detected, as well as low-frequency SNPs.
Discover new variants: compared with the chip method, new variant sequences can be detected.
High cost performance: compared with whole genome de novo sequencing, it takes shorter time and lower cost.
Transcriptome Sequencing
Product Introduction
Transcriptome sequencing is a recently developed technology that uses high-throughput sequencing approaches (next-generation sequencing or NGS) to determine the sequence of all RNA transcripts(mRNA) in a given specimen or specific cell in a certain functional state. Transcriptome sequencing can not only be used for quantitative analysis to detect differences in gene expression levels, but also for structural analysis to find rare transcripts, accurately identify alternative splicing sites, gene fusions, etc. independent of the reference genome.
Product Advantage
Full transcriptome analysis of any species: without needing to design specific probes in advance or know the species gene or genome information, the most comprehensive transcriptome analysis of any species can be directly performed
High coverage: digital signal, sequence almost all transcript fragments directly
Detection threshold range: the range of detection threshold includes 6 orders of magnitude, accurately counts from a few to hundreds of thousands of copies
High resolution: similar genes in gene families and single base differences caused by alternative splicing can be detected
Wide detection range: accurately count from a few to hundreds of thousands of copies, simultaneously normal and rare transcripts can be identified and quantified.
Single cell sequencing
Overview of Single Cell RNA Sequencing Technology
Currently, high-throughput sequencing technology has penetrated into traditional biological research fields such as human diseases, species evolution, and molecular breeding of animal and plant, and has gradually become an indispensable research tool. However, with the in-depth development of life sciences and basic medical research, more and more special specimens and specific biological phenomena have been discovered, such as trace specimens identified by forensic medicine, internal tumor heterogeneity, etc., which cannot be performed by conventional tissue sequencing methods. The emergence of single-cell sequencing technology has brought great convenience to the research of such samples.
©DNBelab C4 scRNA Seq, based on the unique DNBelab C series single cell library preparation technology and powerful DNBSEQ sequencing technology, is composed of DNBelab C4 portable single cell system, DNBelab C series single cell RNA library preparation set, DNBSEQ series sequencing platform, and related single-cell analysis software. DNBelab C4 scRNA Seq can realize the whole process of one-stop single-cell omics research conveniently and real-timely.
Research Workflow
Metagenomics Sequencing
Product Introduction
Microorganisms are the biological group with the largest known species, the largest number, and the widest distribution on the earth. The total number of prokaryotic microorganisms alone is about 4×1030-6×1030. However, traditional methods of separation and culture limit the horizons of understanding the world of microorganisms. It is estimated that more than 99% of the microorganisms in the natural environment cannot be purely cultured by traditional methods, so it is impossible to carry out biotechnology or basic research on them that rely on pure culture. In order to overcome the shortcomings of traditional pure culture techniques and fully tap the huge potential of such uncultivated microorganisms, researchers have developed metagenomics research methods. Using molecular biology research methods to bypass pure culture technology to study the diversity and function of microorganisms provides a culture-free method to explore the diverse structure and functional genome of microorganisms, and is a new way to find new genes and their products.
Content
Metagenomics is the study of all microbial communities in their original living places. Metagenome sequencing refers to sequencing the entire genomes of all microbes present in a sample in order to get the saturated data volume of a single sample, anlysis the diversity of microbial community structure and the genetic composition of microbial populations and metabolic pathways related to specific environments based on de novo assembly. So as to further explore and study the genes with application value and the interrelationships in the microbial communities, or between microbes and the environment. The constructed environmental microbial gene set can provide a gene resource library for the research, development and utilization of microbes in the environment.
Product Advantage
High cost performance: Independent sequencing platform, cost controllable
High sequencing accuracy: DNB sequencing library construction based on DNBSEQ platform rolling circle amplification, PCR amplification errors will not be accumulated, high-fidelity sequence information
Low Duplication rate: low duplication rate of DNBSEQ platform, there are 3%-17% more valid data of the same amount of sequence data
No index hopping worries: no index hopping worries of DNBSEQ platform, and the results are more reliable
For more sequencing services, please consult and contact:
Phone:0755-33945586
Email: CNGBdb@cngb.org