Related data information

Description
Here, we used single cell RNA-sequencing (scRNA-seq) to profile pluripotent stem cell derived human definitive endoderm and intestinal organoids (HIOs) at several timepoints of in vitro growth (7, 14, and 28 days) and after in vivo growth beneath the kidney capsule of a murine host (4 and 8 wks post-transplant). Additionally, we profiled HIOs grown in a non-adhesive alginate hydrogel and also CDX2 knockout HIOs. In order to benchmark the organoid cultures, we used scRNA-seq to profile primary human fetal esophagus (14.3 pcw, 16.7 pcw), stomach (6.7, 14.3, and 16.7 pcw), liver (14.4 pcw), small intestine ( 11.4 and 14.4 pcw) and colon (11.4, 14.4, and 18.9 pcw). Diverse cell lineages were captured across all tissues profiled, including: epithelium, mesenchyme, neurons, endothelium, and immune lineages. development or differentiation design stimulus; time stimulus; time

Taxon
Primates

Species

Tissues
Intestine, Brain, Stem Cells, Kidney, Organoid

Dataset characteristics
Genotype, Cell Type, Tissue, Developmental Stage, Time-Point

Sample number
109

PMID:34019796

Update date: 2021-05-21

Contributors

Emily Holloway; Jason Spence; Qianhui Yu; Joshua Wu