Profiling spatiotemporal gene expression of the developing human spinal cord and implications for ependymoma origin.
IF: 28.771
Cited by: 2


The spatiotemporal regulation of cell fate specification in the human developing spinal cord remains largely unknown. In this study, by performing integrated analysis of single-cell and spatial multi-omics data, we used 16 prenatal human samples to create a comprehensive developmental cell atlas of the spinal cord during post-conceptional weeks 5-12. This revealed how the cell fate commitment of neural progenitor cells and their spatial positioning are spatiotemporally regulated by specific gene sets. We identified unique events in human spinal cord development relative to rodents, including earlier quiescence of active neural stem cells, differential regulation of cell differentiation and distinct spatiotemporal genetic regulation of cell fate choices. In addition, by integrating our atlas with pediatric ependymomas data, we identified specific molecular signatures and lineage-specific genes of cancer stem cells during progression. Thus, we delineate spatiotemporal genetic regulation of human spinal cord development and leverage these data to gain disease insight.


Spatial Temporal Gene Expression
Spatial Temporal Omics

MeSH terms

Spinal Cord
Cell Differentiation
Neural Stem Cells
Gene Expression
Gene Expression Profiling
Gene Expression Regulation, Developmental


Li, Xiaofei
Andrusivova, Zaneta
Czarnewski, Paulo
Langseth, Christoffer Mattsson
Andersson, Alma
Liu, Yang
Gyllborg, Daniel
Braun, Emelie
Larsson, Ludvig
Hu, Lijuan
Alekseenko, Zhanna
Lee, Hower
Avenel, Christophe
Kallner, Helena Kopp
Åkesson, Elisabet
Adameyko, Igor
Nilsson, Mats
Linnarsson, Sten
Lundeberg, Joakim
Sundström, Erik