A spatially resolved atlas of the human lung characterizes a gland-associated immune niche.
IF: 41.307
Cited by: 13


Single-cell transcriptomics has allowed unprecedented resolution of cell types/states in the human lung, but their spatial context is less well defined. To (re)define tissue architecture of lung and airways, we profiled five proximal-to-distal locations of healthy human lungs in depth using multi-omic single cell/nuclei and spatial transcriptomics (queryable at lungcellatlas.org ). Using computational data integration and analysis, we extend beyond the suspension cell paradigm and discover macro and micro-anatomical tissue compartments including previously unannotated cell types in the epithelial, vascular, stromal and nerve bundle micro-environments. We identify and implicate peribronchial fibroblasts in lung disease. Importantly, we discover and validate a survival niche for IgA plasma cells in the airway submucosal glands (SMG). We show that gland epithelial cells recruit B cells and IgA plasma cells, and promote longevity and antibody secretion locally through expression of CCL28, APRIL and IL-6. This new 'gland-associated immune niche' has implications for respiratory health.


Spatial Transcriptomics

MeSH terms

Respiratory Mucosa
Epithelial Cells
Immunoglobulin A


Madissoon, Elo
Oliver, Amanda J
Kleshchevnikov, Vitalii
Wilbrey-Clark, Anna
Polanski, Krzysztof
Richoz, Nathan
Ribeiro Orsi, Ana
Mamanova, Lira
Bolt, Liam
Elmentaite, Rasa
Pett, J Patrick
Huang, Ni
Xu, Chuan
He, Peng
Dabrowska, Monika
Pritchard, Sophie
Tuck, Liz
Prigmore, Elena
Perera, Shani
Knights, Andrew
Oszlanczi, Agnes
Hunter, Adam
Vieira, Sara F
Patel, Minal
Lindeboom, Rik G H
Campos, Lia S
Matsuo, Kazuhiko
Nakayama, Takashi
Yoshida, Masahiro
Worlock, Kaylee B
Nikolić, Marko Z
Georgakopoulos, Nikitas
Mahbubani, Krishnaa T
Saeb-Parsy, Kourosh
Bayraktar, Omer Ali
Clatworthy, Menna R
Stegle, Oliver
Kumasaka, Natsuhiko
Teichmann, Sarah A
Meyer, Kerstin B