A spatiotemporally resolved single-cell atlas of the Plasmodium liver stage.
IF: 69.504
Cited by: 8


Malaria infection involves an obligatory, yet clinically silent liver stage1,2. Hepatocytes operate in repeating units termed lobules, exhibiting heterogeneous gene expression patterns along the lobule axis3, but the effects of hepatocyte zonation on parasite development at the molecular level remain unknown. Here we combine single-cell RNA sequencing4 and single-molecule transcript imaging5 to characterize the host and parasite temporal expression programmes in a zonally controlled manner for the rodent malaria parasite Plasmodium berghei ANKA. We identify differences in parasite gene expression in distinct zones, including potentially co-adaptive programmes related to iron and fatty acid metabolism. We find that parasites develop more rapidly in the pericentral lobule zones and identify a subpopulation of periportally biased hepatocytes that harbour abortive infections, reduced levels of Plasmodium transcripts and parasitophorous vacuole breakdown. These 'abortive hepatocytes', which appear predominantly with high parasite inoculum, upregulate immune recruitment and key signalling programmes. Our study provides a resource for understanding the liver stage of Plasmodium infection at high spatial resolution and highlights the heterogeneous behaviour of both the parasite and the host hepatocyte.


Gene Expression

MeSH terms

Plasmodium berghei
Single-Cell Analysis
Single Molecule Imaging
Sequence Analysis, RNA
Gene Expression Regulation
Fatty Acids
Transcription, Genetic
Genes, Protozoan
Host-Parasite Interactions


Afriat, Amichay
Zuzarte-Luís, Vanessa
Bahar Halpern, Keren
Buchauer, Lisa
Marques, Sofia
Chora, Ângelo Ferreira
Lahree, Aparajita
Amit, Ido
Mota, Maria M
Itzkovitz, Shalev