Resident macrophage subpopulations occupy distinct microenvironments in the kidney.
IF: 9.484
Cited by: 3


The kidney contains a population of resident macrophages from birth that expands as it grows and forms a contiguous network throughout the tissue. Kidney-resident macrophages (KRMs) are important in homeostasis and the response to acute kidney injury. While the kidney contains many microenvironments, it is unknown whether KRMs are a heterogeneous population differentiated by function and location. We combined single-cell RNA-Seq (scRNA-Seq), spatial transcriptomics, flow cytometry, and immunofluorescence imaging to localize, characterize, and validate KRM populations during quiescence and following 19 minutes of bilateral ischemic kidney injury. scRNA-Seq and spatial transcriptomics revealed 7 distinct KRM subpopulations, which are organized into zones corresponding to regions of the nephron. Each subpopulation was identifiable by a unique transcriptomic signature, suggesting distinct functions. Specific protein markers were identified for 2 clusters, allowing analysis by flow cytometry or immunofluorescence imaging. Following injury, the original localization of each subpopulation was lost, either from changing locations or transcriptomic signatures. The original spatial distribution of KRMs was not fully restored for at least 28 days after injury. The change in KRM localization confirmed a long-hypothesized dysregulation of the local immune system following acute injury and may explain the increased risk for chronic kidney disease.


Spatial Transcriptomics
Innate immunity

MeSH terms

Acute Kidney Injury
Flow Cytometry
Renal Insufficiency, Chronic


Cheung, Matthew D
Erman, Elise N
Moore, Kyle H
Lever, Jeremie Mp
Li, Zhang
LaFontaine, Jennifer R
Ghajar-Rahimi, Gelare
Liu, Shanrun
Yang, Zhengqin
Karim, Rafay
Yoder, Bradley K
Agarwal, Anupam
George, James F