Decoding the olfactory map through targeted transcriptomics links murine olfactory receptors to glomeruli.
IF: 17.694
Cited by: 7


Sensory processing in olfactory systems is organized across olfactory bulb glomeruli, wherein axons of peripheral sensory neurons expressing the same olfactory receptor co-terminate to transmit receptor-specific activity to central neurons. Understanding how receptors map to glomeruli is therefore critical to understanding olfaction. High-throughput spatial transcriptomics is a rapidly advancing field, but low-abundance olfactory receptor expression within glomeruli has previously precluded high-throughput mapping of receptors to glomeruli in the mouse. Here we combined sequential sectioning along the anteroposterior, dorsoventral, and mediolateral axes with target capture enrichment sequencing to overcome low-abundance target expression. This strategy allowed us to spatially map 86% of olfactory receptors across the olfactory bulb and uncover a relationship between OR sequence and glomerular position.


Spatial Transcriptomics

MeSH terms

Olfactory Bulb
Olfactory Receptor Neurons
Receptors, Odorant


Zhu, Kevin W
Burton, Shawn D
Nagai, Maira H
Silverman, Justin D
de March, Claire A
Wachowiak, Matt
Matsunami, Hiroaki