Single-cell Stereo-seq reveals induced progenitor cells involved in axolotl brain regeneration.
IF: 63.714
Cited by: 46


The molecular mechanism underlying brain regeneration in vertebrates remains elusive. We performed spatial enhanced resolution omics sequencing (Stereo-seq) to capture spatially resolved single-cell transcriptomes of axolotl telencephalon sections during development and regeneration. Annotated cell types exhibited distinct spatial distribution, molecular features, and functions. We identified an injury-induced ependymoglial cell cluster at the wound site as a progenitor cell population for the potential replenishment of lost neurons, through a cell state transition process resembling neurogenesis during development. Transcriptome comparisons indicated that these induced cells may originate from local resident ependymoglial cells. We further uncovered spatially defined neurons at the lesion site that may regress to an immature neuron-like state. Our work establishes spatial transcriptome profiles of an anamniote tetrapod brain and decodes potential neurogenesis from ependymoglial cells for development and regeneration, thus providing mechanistic insights into vertebrate brain regeneration.


Spatial Omics

MeSH terms

Ambystoma mexicanum
Brain Regeneration
Neural Stem Cells
Single-Cell Analysis


Wei, Xiaoyu
Fu, Sulei
Li, Hanbo
Liu, Yang
Wang, Shuai
Feng, Weimin
Yang, Yunzhi
Liu, Xiawei
Zeng, Yan-Yun
Cheng, Mengnan
Lai, Yiwei
Qiu, Xiaojie
Wu, Liang
Zhang, Nannan
Jiang, Yujia
Xu, Jiangshan
Su, Xiaoshan
Peng, Cheng
Han, Lei
Lou, Wilson Pak-Kin
Liu, Chuanyu
Yuan, Yue
Ma, Kailong
Yang, Tao
Pan, Xiangyu
Gao, Shang
Chen, Ao
Esteban, Miguel A
Yang, Huanming
Wang, Jian
Fan, Guangyi
Liu, Longqi
Chen, Liang
Xu, Xun
Fei, Ji-Feng
Gu, Ying