Spatial multi-omic map of human myocardial infarction.
IF: 69.504
Cited by: 98


Myocardial infarction is a leading cause of death worldwide1. Although advances have been made in acute treatment, an incomplete understanding of remodelling processes has limited the effectiveness of therapies to reduce late-stage mortality2. Here we generate an integrative high-resolution map of human cardiac remodelling after myocardial infarction using single-cell gene expression, chromatin accessibility and spatial transcriptomic profiling of multiple physiological zones at distinct time points in myocardium from patients with myocardial infarction and controls. Multi-modal data integration enabled us to evaluate cardiac cell-type compositions at increased resolution, yielding insights into changes of the cardiac transcriptome and epigenome through the identification of distinct tissue structures of injury, repair and remodelling. We identified and validated disease-specific cardiac cell states of major cell types and analysed them in their spatial context, evaluating their dependency on other cell types. Our data elucidate the molecular principles of human myocardial tissue organization, recapitulating a gradual cardiomyocyte and myeloid continuum following ischaemic injury. In sum, our study provides an integrative molecular map of human myocardial infarction, represents an essential reference for the field and paves the way for advanced mechanistic and therapeutic studies of cardiac disease.


Spatial Transcriptomics

MeSH terms

Atrial Remodeling
Case-Control Studies
Chromatin Assembly and Disassembly
Gene Expression Profiling
Myocardial Infarction
Myocytes, Cardiac
Single-Cell Analysis
Time Factors
Ventricular Remodeling


Kuppe, Christoph
Ramirez Flores, Ricardo O
Li, Zhijian
Hayat, Sikander
Levinson, Rebecca T
Liao, Xian
Hannani, Monica T
Tanevski, Jovan
Wünnemann, Florian
Nagai, James S
Halder, Maurice
Schumacher, David
Menzel, Sylvia
Schäfer, Gideon
Hoeft, Konrad
Cheng, Mingbo
Ziegler, Susanne
Zhang, Xiaoting
Peisker, Fabian
Kaesler, Nadine
Saritas, Turgay
Xu, Yaoxian
Kassner, Astrid
Gummert, Jan
Morshuis, Michiel
Amrute, Junedh
Veltrop, Rogier J A
Boor, Peter
Klingel, Karin
Van Laake, Linda W
Vink, Aryan
Hoogenboezem, Remco M
Bindels, Eric M J
Schurgers, Leon
Sattler, Susanne
Schapiro, Denis
Schneider, Rebekka K
Lavine, Kory
Milting, Hendrik
Costa, Ivan G
Saez-Rodriguez, Julio
Kramann, Rafael