Single-cell and spatial mapping Identify cell types and signaling Networks in the human ureter.
IF: 13.417
Cited by: 4


Tissue engineering offers a promising treatment strategy for ureteral strictures, but its success requires an in-depth understanding of the architecture, cellular heterogeneity, and signaling pathways underlying tissue regeneration. Here, we define and spatially map cell populations within the human ureter using single-cell RNA sequencing, spatial gene expression, and immunofluorescence approaches. We focus on the stromal and urothelial cell populations to enumerate the distinct cell types composing the human ureter and infer potential cell-cell communication networks underpinning the bi-directional crosstalk between these compartments. Furthermore, we analyze and experimentally validate the importance of the sonic hedgehog (SHH) signaling pathway in adult progenitor cell maintenance. The SHH-expressing basal cells support organoid generation in vitro and accurately predict the differentiation trajectory from basal progenitor cells to terminally differentiated umbrella cells. Our results highlight the essential processes involved in adult ureter tissue homeostasis and provide a blueprint for guiding ureter tissue engineering.


Spatial Transcriptomics
Spatial Gene Expression
adult stem cell
human ureter
single-cell RNA sequencing
sonic hedgehog
spatial gene expression
tissue engineering

MeSH terms

Cell Differentiation
Hedgehog Proteins
Signal Transduction
Stem Cells


Fink, Emily E
Sona, Surbhi
Tran, Uyen
Desprez, Pierre-Emmanuel
Bradley, Matthew
Qiu, Hong
Eltemamy, Mohamed
Wee, Alvin
Wolkov, Madison
Nicolas, Marlo
Min, Booki
Haber, Georges-Pascal
Wessely, Oliver
Lee, Byron H
Ting, Angela H