Spatially resolved transcriptomics and the kidney: many opportunities.
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IF: 18.998
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Abstract

Defining changes in gene expression during health and disease is critical for the understanding of human physiology. In recent years, single-cell/nuclei RNA sequencing (sc/snRNAseq) has revolutionized the definition and discovery of cell types and states as well as the interpretation of organ- and cell-type-specific signaling pathways. However, these advances require tissue dissociation to the level of the single cell or single nuclei level. Spatially resolved transcriptomics (SrT) now provides a platform to overcome this barrier in understanding the physiological contexts of gene expression and cellular microenvironment changes in development and disease. Some of these transcriptomic tools allow for high-resolution mapping of hundreds of genes simultaneously in cellular and subcellular compartments. Other tools offer genome depth mapping but at lower resolution. We review advances in SrT, considerations for using SrT in your own research, and applications for kidney biology.

Keywords

Seurat
Spatial Transcriptomics
seqFISH+
MERFISH
smFISH
RNAscope
SM-Omics
Slide-seq
Tomo-seq
SRM
ISS
Spatial reconstruction
INSTA-seq
HDST
pciSeq
cell signaling
histology
transcription regulation

MeSH terms

Cellular Microenvironment
Gene Expression Profiling
Humans
Kidney
Transcriptome

Authors

Dixon, Eryn E
Wu, Haojia
Sulvarán-Guel, Elizabeth
Guo, Juanru
Humphreys, Benjamin D