Identification of two cancer stem cell-like populations in triple-negative breast cancer xenografts.
IF: 5.732


Gene expression analysis at the single-cell level by next-generation sequencing has revealed the existence of clonal dissemination and microheterogeneity in cancer metastasis. The current spatial analysis technologies can elucidate the heterogeneity of cell-cell interactions in situ. To reveal the regional and expressional heterogeneity in primary tumors and metastases, we performed transcriptomic analysis of microtissues dissected from a triple-negative breast cancer (TNBC) cell line MDA-MB-231 xenograft model with our automated tissue microdissection punching technology. This multiple-microtissue transcriptome analysis revealed three cancer cell-type clusters in the primary tumor and axillary lymph node metastasis, two of which were cancer stem cell (CSC)-like clusters (CD44/MYC-high, HMGA1-high). Reanalysis of public single-cell RNA-sequencing datasets confirmed that the two CSC-like populations existed in TNBC xenograft models and in TNBC patients. The diversity of these multiple CSC-like populations could cause differential anticancer drug resistance, increasing the difficulty of curing this cancer.


Spatial Transcriptomics
Gene Expression
Breast cancer
Cancer stem cell
Spatial transcriptome
Xenograft model

MeSH terms

Antineoplastic Agents
Cell Line, Tumor
Neoplastic Stem Cells
Triple Negative Breast Neoplasms


Nakayama, Jun
Matsunaga, Hiroko
Arikawa, Koji
Yoda, Takuya
Hosokawa, Masahito
Takeyama, Haruko
Yamamoto, Yusuke
Semba, Kentaro