The single-cell stereo-seq reveals region-specific cell subtypes and transcriptome profiling in Arabidopsis leaves.
IF: 13.417
Cited by: 60


Understanding the complex functions of plant leaves requires a thorough characterization of discrete cell features. Although single-cell gene expression profiling technologies have been developed, their application in characterizing cell subtypes has not been achieved yet. Here, we present scStereo-seq (single-cell spatial enhanced resolution omics sequencing) that enabled us to show the bona fide single-cell spatial transcriptome profiles of Arabidopsis leaves. Subtle but significant transcriptomic differences between upper and lower epidermal cells have been successfully distinguished. Furthermore, we discovered cell-type-specific gene expression gradients from the main vein to the leaf edge, which led to the finding of distinct spatial developmental trajectories of vascular cells and guard cells. Our study showcases the importance of physical locations of individual cells for exerting complex biological functions in plants and demonstrates that scStereo-seq is a powerful tool to integrate single-cell location and transcriptome information for plant biology study.


Spatial Omics
Spatial Gene Expression
Arabidopsis leaves
single-cell Stereo-seq
single-cell spatial transcriptome
spatial developmental trajectory


Xia, Keke
Sun, Hai-Xi
Li, Jie
Li, Jiming
Zhao, Yu
Chen, Lichuan
Qin, Chao
Chen, Ruiying
Chen, Zhiyong
Liu, Guangyu
Yin, Ruilian
Mu, Bangbang
Wang, Xiaojuan
Xu, Mengyuan
Li, Xinyue
Yuan, Peisi
Qiao, Yixin
Hao, Shijie
Wang, Jing
Xie, Qing
Xu, Jiangshan
Liu, Shiping
Li, Yuxiang
Chen, Ao
Liu, Longqi
Yin, Ye
Yang, Huanming
Wang, Jian
Gu, Ying
Xu, Xun

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