Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons.
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IF: 8.713
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Cited by: 77
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Abstract

Somatosensory neurons with cell bodies in the dorsal root ganglia (DRG) project to the skin, muscles, bones, and viscera to detect touch and temperature as well as to mediate proprioception and many types of interoception. In addition, the somatosensory system conveys the clinically relevant noxious sensations of pain and itch. Here, we used single nuclear transcriptomics to characterize transcriptomic classes of human DRG neurons that detect these diverse types of stimuli. Notably, multiple types of human DRG neurons have transcriptomic features that resemble their mouse counterparts although expression of genes considered important for sensory function often differed between species. More unexpectedly, we identified several transcriptomic classes with no clear equivalent in the other species. This dataset should serve as a valuable resource for the community, for example as means of focusing translational efforts on molecules with conserved expression across species.

Keywords

Seurat
Gene Expression
Omics
Spatial Transcriptomics
RNAscope
dorsal root ganglia
human
itch
mouse
neuroscience
pain
somatosensation
transcriptomics

MeSH terms

Adult
Animals
Cell Nucleus
Female
Ganglia, Spinal
Gene Expression Profiling
Humans
Male
Mice
Middle Aged
Neurons
Single-Cell Analysis
Transcriptome

Authors

Nguyen, Minh Q
von Buchholtz, Lars J
Reker, Ashlie N
Ryba, Nicholas Jp
Davidson, Steve

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