Transcriptional landscape of highly lignified poplar stems at single-cell resolution.
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IF: 17.906
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Cited by: 27
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Abstract

Plant secondary growth depends on the activity of the vascular cambium, which produces xylem and phloem. Wood derived from xylem is the most abundant form of biomass globally and has played key socio-economic and subsistence roles throughout human history. However, despite intensive study of vascular development, the full diversity of cell types and the gene networks engaged are still poorly understood. Here, we have applied an optimized protoplast isolation protocol and RNA sequencing to characterize the high-resolution single-cell transcriptional landscape of highly lignified poplar stems. We identify 20 putative cell clusters with a series of novel cluster-specific marker genes and find that these cells are highly heterogeneous based on the transcriptome. Analysis of these marker genes' expression dynamics enables reconstruction of the cell differentiation trajectories involved in phloem and xylem development. We find that different cell clusters exhibit distinct patterns of phytohormone responses and emphasize the use of our data to predict potential gene redundancy and identify candidate genes related to vascular development in trees. These findings establish the transcriptional landscape of major cell types of poplar stems at single-cell resolution and provide a valuable resource for investigating basic principles of vascular cell specification and differentiation in trees.

Keywords

Seurat
LCM-seq
Spatial Transcriptomics
Gene Expression

MeSH terms

Biomass
Cambium
Gene Expression Regulation, Plant
Genetic Markers
Multigene Family
Phloem
Plant Growth Regulators
Plant Proteins
Plant Stems
Populus
RNA-Seq
Single-Cell Analysis
Transcriptome
Trees
Xylem

Authors

Chen, Yang
Tong, Shaofei
Jiang, Yuanzhong
Ai, Fandi
Feng, Yanlin
Zhang, Junlin
Gong, Jue
Qin, Jiajia
Zhang, Yuanyuan
Zhu, Yingying
Liu, Jianquan
Ma, Tao

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