Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
IF: 31.373
Cited by: 74


Mitochondria are key organelles for cellular energetics, metabolism, signaling, and quality control and have been linked to various diseases. Different views exist on the composition of the human mitochondrial proteome. We classified >8,000 proteins in mitochondrial preparations of human cells and defined a mitochondrial high-confidence proteome of >1,100 proteins (MitoCoP). We identified interactors of translocases, respiratory chain, and ATP synthase assembly factors. The abundance of MitoCoP proteins covers six orders of magnitude and amounts to 7% of the cellular proteome with the chaperones HSP60-HSP10 being the most abundant mitochondrial proteins. MitoCoP dynamics spans three orders of magnitudes, with half-lives from hours to months, and suggests a rapid regulation of biosynthesis and assembly processes. 460 MitoCoP genes are linked to human diseases with a strong prevalence for the central nervous system and metabolism. MitoCoP will provide a high-confidence resource for placing dynamics, functions, and dysfunctions of mitochondria into the cellular context.


Spatial Proteomics
copy numbers
high-confidence proteome
human cells
protein translocation
respiratory chain


Morgenstern, Marcel
Peikert, Christian D
Lübbert, Philipp
Suppanz, Ida
Klemm, Cinzia
Alka, Oliver
Steiert, Conny
Naumenko, Nataliia
Schendzielorz, Alexander
Melchionda, Laura
Mühlhäuser, Wignand W D
Knapp, Bettina
Busch, Jakob D
Stiller, Sebastian B
Dannenmaier, Stefan
Lindau, Caroline
Licheva, Mariya
Eickhorst, Christopher
Galbusera, Riccardo
Zerbes, Ralf M
Ryan, Michael T
Kraft, Claudine
Kozjak-Pavlovic, Vera
Drepper, Friedel
Dennerlein, Sven
Oeljeklaus, Silke
Pfanner, Nikolaus
Wiedemann, Nils
Warscheid, Bettina

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