Advances in single-cell sequencing: insights from organ transplantation.
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IF: 34.915
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Cited by: 1
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Abstract

Single-cell RNA sequencing (scRNA-seq) is a comprehensive technical tool to analyze intracellular and intercellular interaction data by whole transcriptional profile analysis. Here, we describe the application in biomedical research, focusing on the immune system during organ transplantation and rejection. Unlike conventional transcriptome analysis, this method provides a full map of multiple cell populations in one specific tissue and presents a dynamic and transient unbiased method to explore the progression of allograft dysfunction, starting from the stress response to final graft failure. This promising sequencing technology remarkably improves individualized organ rejection treatment by identifying decisive cellular subgroups and cell-specific interactions.

Keywords

LCM-seq
Omics
Seurat
Spatial Transcriptomics
Gene Expression
smFISH
10× Genomics chromium
Immune cell interactions
Single-cell RNA sequencing
Transcriptional profiling
Transplant rejection

MeSH terms

Gene Expression Profiling
Humans
Organ Transplantation
Sequence Analysis, RNA
Single-Cell Analysis

Authors

Wang, Ying
Wang, Jian-Ye
Schnieke, Angelika
Fischer, Konrad

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