Advances in single-cell sequencing: insights from organ transplantation.

IF: 34.915

Cited by: 1

Abstract

Single-cell RNA sequencing (scRNA-seq) is a comprehensive technical tool to analyze intracellular and intercellular interaction data by whole transcriptional profile analysis. Here, we describe the application in biomedical research, focusing on the immune system during organ transplantation and rejection. Unlike conventional transcriptome analysis, this method provides a full map of multiple cell populations in one specific tissue and presents a dynamic and transient unbiased method to explore the progression of allograft dysfunction, starting from the stress response to final graft failure. This promising sequencing technology remarkably improves individualized organ rejection treatment by identifying decisive cellular subgroups and cell-specific interactions.

Keywords

smFISH

Seurat

Gene Expression

Omics

Spatial Transcriptomics

LCM-seq

10× Genomics chromium

Immune cell interactions

Single-cell RNA sequencing

Transcriptional profiling

Transplant rejection

MeSH terms

Gene Expression Profiling

Humans

Organ Transplantation

Sequence Analysis, RNA

Single-Cell Analysis

Authors

Wang, Ying

Wang, Jian-Ye

Schnieke, Angelika

Fischer, Konrad

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