Molecular characterization of projection neuron subtypes in the mouse olfactory bulb.
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IF: 8.713
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Cited by: 1
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Abstract

Projection neurons (PNs) in the mammalian olfactory bulb (OB) receive input from the nose and project to diverse cortical and subcortical areas. Morphological and physiological studies have highlighted functional heterogeneity, yet no molecular markers have been described that delineate PN subtypes. Here, we used viral injections into olfactory cortex and fluorescent nucleus sorting to enrich PNs for high-throughput single nucleus and bulk RNA deep sequencing. Transcriptome analysis and RNA in situ hybridization identified distinct mitral and tufted cell populations with characteristic transcription factor network topology, cell adhesion, and excitability-related gene expression. Finally, we describe a new computational approach for integrating bulk and snRNA-seq data and provide evidence that different mitral cell populations preferentially project to different target regions. Together, we have identified potential molecular and gene regulatory mechanisms underlying PN diversity and provide new molecular entry points into studying the diverse functional roles of mitral and tufted cell subtypes.

Keywords

Seurat
RNAscope
smFISH
Gene Expression
PROCEDURE
cell type
mouse
neuroscience
olfaction
projection neuron
transcription factor network
transcriptome

MeSH terms

Animals
Female
Interneurons
Male
Mice
Mice, Inbred C57BL
Neurons
Olfactory Bulb
Sequence Analysis, RNA
Transcription Factors

Authors

Zeppilli, Sara
Ackels, Tobias
Attey, Robin
Klimpert, Nell
Ritola, Kimberly D
Boeing, Stefan
Crombach, Anton
Schaefer, Andreas T
Fleischmann, Alexander

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