High-resolution characterization of gene function using single-cell CRISPR tiling screen.
IF: 17.694
Cited by: 1


Identification of novel functional domains and characterization of detailed regulatory mechanisms in cancer-driving genes is critical for advanced cancer therapy. To date, CRISPR gene editing has primarily been applied to defining the role of individual genes. Recently, high-density mutagenesis via CRISPR tiling of gene-coding exons has been demonstrated to identify functional regions in genes. Furthermore, breakthroughs in combining CRISPR library screens with single-cell droplet RNA sequencing (sc-RNAseq) platforms have revealed the capacity to monitor gene expression changes upon genetic perturbations at single-cell resolution. Here, we present "sc-Tiling," which integrates a CRISPR gene-tiling screen with single-cell transcriptomic and protein structural analyses. Distinct from other reported single-cell CRISPR screens focused on observing gene function and gene-to-gene/enhancer-to-gene regulation, sc-Tiling enables the capacity to identify regulatory mechanisms within a gene-coding region that dictate gene activity and therapeutic response.


Gene Expression

MeSH terms

CRISPR-Cas Systems
Clustered Regularly Interspaced Short Palindromic Repeats
Drug Screening Assays, Antitumor
Gene Editing
Gene Expression Regulation, Neoplastic
Genetic Testing
Genome, Human
Histone-Lysine N-Methyltransferase
Models, Molecular


Yang, Lu
Chan, Anthony K N
Miyashita, Kazuya
Delaney, Christopher D
Wang, Xi
Li, Hongzhi
Pokharel, Sheela Pangeni
Li, Sandra
Li, Mingli
Xu, Xiaobao
Lu, Wei
Liu, Qiao
Mattson, Nicole
Chen, Kevin Yining
Wang, Jinhui
Yuan, Yate-Ching
Horne, David
Rosen, Steven T
Soto-Feliciano, Yadira
Feng, Zhaohui
Hoshii, Takayuki
Xiao, Gang
Müschen, Markus
Chen, Jianjun
Armstrong, Scott A
Chen, Chun-Wei

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