deepBlink: threshold-independent detection and localization of diffraction-limited spots.
Abstract
Detection of diffraction-limited spots in single-molecule microscopy images is traditionally performed with mathematical operators designed for idealized spots. This process requires manual tuning of parameters that is time-consuming and not always reliable. We have developed deepBlink, a neural network-based method to detect and localize spots automatically. We demonstrate that deepBlink outperforms other state-of-the-art methods across six publicly available datasets containing synthetic and experimental data.
Keywords
smFISH
Spatial Transcriptomics
MeSH terms
Image Processing, Computer-Assisted
Microscopy
Neural Networks, Computer
Software
Authors
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