In Situ Localization of Plant Lipid Metabolites by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI-MSI).

PMID:34047991

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Abstract

Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has emerged as a major analytical platform for the determination and localization of lipid metabolites directly from tissue sections. Unlike analysis of lipid extracts, where lipid localizations are lost due to homogenization and/ or solvent extraction, MALDI-MSI analysis is capable of revealing spatial localization of metabolites while simultaneously collecting high chemical resolution mass spectra. Important considerations for obtaining high quality MALDI-MS images include tissue preservation, section preparation, MS data collection and data processing. Errors in any of these steps can lead to poor quality metabolite images and increases the chance for metabolite misidentification and/ or incorrect localization. Here, we present detailed methods and recommendations for specimen preparation, MALDI-MS instrument parameters, software analysis platforms for data processing, and practical considerations for each of these steps to ensure acquisition of high-quality chemical and spatial resolution data for reconstructing MALDI-MS images of plant tissues.

Keywords

MALDI

MALDI-MSI

Camelina sativa

Data processing

Lipids

MALDI mass spectrometry imaging

Phosphatidylcholine

Plant tissues

Seeds

Triacylglycerol

MeSH terms

Diagnostic Imaging

Histological Techniques

Image Processing, Computer-Assisted

Lipid Metabolism

Lipids

Plants

Software

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Authors

Sturtevant, Drew

Aziz, Mina

Romsdahl, Trevor B

Corley, Chase D

Chapman, Kent D

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