Spatial multi-omics sequencing for fixed tissue via DBiT-seq.
This protocol describes the use of the deterministic barcoding in tissue for spatial omics sequencing platform to construct a multi-omics atlas on fixed frozen tissue samples. This approach uses a microfluidic-based method to introduce combinatorial DNA oligo barcodes directly to the cells in a tissue section fixed on a glass slide. This technique does not directly resolve single cells but can achieve a near-single-cell resolution for spatial transcriptomics and spatial analysis of a targeted panel of proteins. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).
DNA Barcoding, Taxonomic
Gene Expression Profiling
Microfluidic Analytical Techniques
Sequence Analysis, DNA
1. High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue.
2. Spatial transcriptomics of the nematode Caenorhabditis elegans using RNA tomography.
3. Probing infectious disease by single-cell RNA sequencing: Progresses and perspectives.
4. Protocol for single-molecule fluorescence recovery after photobleaching microscopy to analyze the dynamics and spatial locations of nuclear transmembrane proteins in live cells.
5. Review of multi-omics data resources and integrative analysis for human brain disorders.
1. High spatial resolution multi-omics atlas sequencing of mouse embryos
2. Seq-Scope: Submicrometer-resolution spatial barcoding technology that enables microscopic examination of tissue transcriptome at single cell and subcellular levels
3. Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia
4. White matter aging drives microglial diversity
5. Photo-isolation chemistry for high-resolution and deep spatial transcriptome with tissue sections