Spatial multi-omics sequencing for fixed tissue via DBiT-seq.
Abstract
This protocol describes the use of the deterministic barcoding in tissue for spatial omics sequencing platform to construct a multi-omics atlas on fixed frozen tissue samples. This approach uses a microfluidic-based method to introduce combinatorial DNA oligo barcodes directly to the cells in a tissue section fixed on a glass slide. This technique does not directly resolve single cells but can achieve a near-single-cell resolution for spatial transcriptomics and spatial analysis of a targeted panel of proteins. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).
Keywords
Spatial Transcriptomics
DBiT-seq
SM-Omics
Genomics
RNA-seq
Sequencing
MeSH terms
DNA
DNA Barcoding, Taxonomic
Equipment Design
Gene Expression Profiling
Genomics
Microfluidic Analytical Techniques
Sequence Analysis, DNA
Transcriptome
Authors
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