A coordinated progression of progenitor cell states initiates urinary tract development.
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IF: 17.694
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Cited by: 16
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Datasets
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Abstract

The kidney and upper urinary tract develop through reciprocal interactions between the ureteric bud and the surrounding mesenchyme. Ureteric bud branching forms the arborized collecting duct system of the kidney, while ureteric tips promote nephron formation from dedicated progenitor cells. While nephron progenitor cells are relatively well characterized, the origin of ureteric bud progenitors has received little attention so far. It is well established that the ureteric bud is induced from the nephric duct, an epithelial duct derived from the intermediate mesoderm of the embryo. However, the cell state transitions underlying the progression from intermediate mesoderm to nephric duct and ureteric bud remain unknown. Here we show that nephric duct morphogenesis results from the coordinated organization of four major progenitor cell populations. Using single cell RNA-seq and Cluster RNA-seq, we show that these progenitors emerge in time and space according to a stereotypical pattern. We identify the transcription factors Tfap2a/b and Gata3 as critical coordinators of this progenitor cell progression. This study provides a better understanding of the cellular origin of the renal collecting duct system and associated urinary tract developmental diseases, which may inform guided differentiation of functional kidney tissue.

Keywords

LCM-seq
Tomo-seq
Seurat
Spatial Transcriptomics
Gene Expression

MeSH terms

Animals
Cell Differentiation
Embryo, Mammalian
Female
GATA3 Transcription Factor
Gene Expression Regulation, Developmental
Male
Mice
Mice, Transgenic
Models, Animal
Nephrons
Organogenesis
RNA-Seq
Single-Cell Analysis
Stem Cells
Transcription Factor AP-2

Authors

Sanchez-Ferras, Oraly
Pacis, Alain
Sotiropoulou, Maria
Zhang, Yuhong
Wang, Yu Chang
Bourgey, Mathieu
Bourque, Guillaume
Ragoussis, Jiannis
Bouchard, Maxime

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