Advances and Opportunities in Single-Cell Transcriptomics for Plant Research.
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IF: 28.310
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Cited by: 80
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Abstract

Single-cell approaches are quickly changing our view on biological systems by increasing the spatiotemporal resolution of our analyses to the level of the individual cell. The field of plant biology has fully embraced single-cell transcriptomics and is rapidly expanding the portfolio of available technologies and applications. In this review, we give an overview of the main advances in plant single-cell transcriptomics over the past few years and provide the reader with an accessible guideline covering all steps, from sample preparation to data analysis. We end by offering a glimpse of how these technologies will shape and accelerate plant-specific research in the near future.

Keywords

Spatial reconstruction
Seurat
seqFISH+
Omics
MERFISH
Spatial Transcriptomics
NICHE-seq
LCM-seq
cell atlas
cell trajectory
plant transcriptomics
protoplast
scRNA-seq

MeSH terms

Computational Biology
Plants
Sequence Analysis, RNA
Single-Cell Analysis
Transcriptome

Authors

Seyfferth, Carolin
Renema, Jim
Wendrich, Jos R
Eekhout, Thomas
Seurinck, Ruth
Vandamme, Niels
Blob, Bernhard
Saeys, Yvan
Helariutta, Yrjo
Birnbaum, Kenneth D
De Rybel, Bert

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