Single-cell transcriptomic analysis of the adult mouse spinal cord reveals molecular diversity of autonomic and skeletal motor neurons.
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IF: 28.771
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Cited by: 89
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Abstract

The spinal cord is a fascinating structure that is responsible for coordinating movement in vertebrates. Spinal motor neurons control muscle activity by transmitting signals from the spinal cord to diverse peripheral targets. In this study, we profiled 43,890 single-nucleus transcriptomes from the adult mouse spinal cord using fluorescence-activated nuclei sorting to enrich for motor neuron nuclei. We identified 16 sympathetic motor neuron clusters, which are distinguishable by spatial localization and expression of neuromodulatory signaling genes. We found surprising skeletal motor neuron heterogeneity in the adult spinal cord, including transcriptional differences that correlate with electrophysiologically and spatially distinct motor pools. We also provide evidence for a novel transcriptional subpopulation of skeletal motor neuron (γ*). Collectively, these data provide a single-cell transcriptional atlas ( http://spinalcordatlas.org ) for investigating the organizing molecular logic of adult motor neuron diversity, as well as the cellular and molecular basis of motor neuron function in health and disease.

Keywords

Seurat
LCM-seq
Spatial Gene Expression

MeSH terms

Animals
Autonomic Nervous System
Mice
Motor Neurons
Muscle, Skeletal
Single-Cell Analysis
Spinal Cord
Transcriptome
Viscera

Authors

Blum, Jacob A
Klemm, Sandy
Shadrach, Jennifer L
Guttenplan, Kevin A
Nakayama, Lisa
Kathiria, Arwa
Hoang, Phuong T
Gautier, Olivia
Kaltschmidt, Julia A
Greenleaf, William J
Gitler, Aaron D

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